Featured Publications
Hall, B, Jones, L, Forrest, J. Competitive effects from an artificial tear solution to protein adsorption. Optometry and Vision Science. 2015; 92(7):781-789.


To compare the adsorption of lysozyme, lactoferrin, and albumin to various contact lens materials, between single-protein solutions and a multicomponent artificial tear solution (ATS). Additionally, extra steps were taken to distinguish loosely and tightly bound protein, the latter of which may be fully or partially denatured.


Using a previously described ATS, we measured the time-dependent adsorption of lys, lac, and alb onto one conventional hydrogel and four silicone hydrogel contact lens materials between the first minute and up to 1 week of protein interaction with the material surface. Proteins were quantified using I125radiolabeling of each protein individually in ATS and buffered saline. Extra steps were taken to limit the amount of unbound I125 and to quantify the amount of reversibly bound protein.


Comfilcon A, balafilcon A, and etafilcon A did not show any relevant competitive adsorption between the ATS components and lys, lac, or alb until after 1 week. Competitive adsorption effects for lys, lac, and alb were observed in as little as 1 minute on lotrafilcon B. Lotrafilcon B had no reversibly bound protein at any time points. The ionic materials balafilcon A and etafilcon A deposited significant amounts of reversibly bound lysozyme and lactoferrin in just 10 minutes. Senofilcon A apparent deposition was below our thresholds of confidence for this protein quantification method.


Both the competition between lys, lac, and alb and ATS components and the reversibility of these bound proteins is material specific. Coadsorption of lys, lac, and alb with ATS components can increase the reversibility of their adsorption.

Download Full Text

Make the Best Choice for your Research

Sengi helps small and medium-sized businesses get brilliant ocular health discoveries into the hands of your ideal customer. You don’t need to have a specialized writer in-house to turn your research into reality.

contact sengi data for reporting testing

Contact Sengi

Please let us know your name.

Please let us know your email address.

Please write a subject for your message.

Please let us know your message.

Invalid Input

sengi footer logo

The Sengi team is led by Dr. Brad Hall, a vision scientist and expert medical writer. A regular peer reviewer for several medical and ophthalmology journals, Dr. Hall has authored a multitude of articles personally, is a successful grant writer, medical writer, and master of the art of simplifying data and statistical analysis. Since launching in 2015, Sengi has provided medical writing and biostatistics analysis expertise to SMBs and researchers around the world that lacked the necessary means to share their scientific breakthroughs outside of the lab. Sengi’s work has enabled these companies to put advanced technology into the hands of those who need them most.